ABSTRACT
Objective To investigate the current status of anti-mullerian hormone (AMH) in healthy women in the healthy childbearing age in Shenzhen area,and to explore its application value in polycystic ovary syndrome (PCOS) and prature ovarian syndrome (POF) treatment.Methods Collected 860 cases of medical health women of childbearing age in Shenzhen BaoanDistrict People's Hospital from January 2016 to March 2017 as control group,and at the same time selected 59 cases of patients with PCOS and 61 cases of patients with POF as observation group.Adopted DXI800 chemiluminescence analyzer to test serum AMH level of control group,patients with PCOS and POF group respectively before and 3 months after treatment,and the testing results were analyzed.Results The serum AMH level of Healthy women of childbearing age was 4.91 ±1.56 ng/ml,18～25 years old group was 5.38±1.27 ng/ml,31～35 and 36～42 years old group were 4.53±1.40 ng/ml and 3.95±1.16 ng/ml,being all lower than that in group 18～25 years old (t=3.082,5.066,all P＜0.05).While 26～30 years old group was 5.09± 1.45 ng/ml,and compared with 18～ 25,there was no statistically significant difference (t=0.892,P＞0.05).Before the treatment,the serum AMH level of PCOS was 10.13± 3.85 ng/ml,significantly higher than the control group,the difference was statistically significant (t=13.924,P＜0.01),while the serum AMH level of POF group and wait POF patients were 1.04±0.37 ng/ml and 2.39±0.87 ng/ml,significantly lower than the control group (t=10.913,8.042,all P＜0.05),and POF lower than wait POF group (t=2.875,P＜0.05).After treatment,the serum AMH level of PCOS was 5.22± 1.58 ng/ml,significantly lower than before treatment (t =11.106,P＜0.05),and there was no statistically significant difference between control group (t=1.036,P＞0.05),the serum AMH level of POF and wait POF were 4.49±1.32 ng/ml and 4.54± 1.47 ng/ml,significantly higher than the before treatment (t=9.608,7.253,all P＜0.05),and compared with the control group there were no statistically significant differences (t =1.209,0.918,all P＞0.05).Conclusion Before the treatment,the serum AMH levels of PCOS were increased significantly,and the serum AMH levels of POF and wait POF patients were significantly reduced,while the serum AMH levels of PCOS,POF and wait POF patients were all restored to normal levels after treatment.Therefore,AMH has certain application value in the diagnosis and treatment evaluation of women PCOS and POF disease.
ABSTRACT
<p><b>AIM</b>To study the effects of Cu2+ on growth hormone (GH) secretion of pig pituitary cells in vivo.</p><p><b>METHODS</b>Gland pituitary cells of 32-35 days old pigs were incubated for 48 h in presence of 10% fetal calf serum in DMEM. Cells were treated for 36 h with various concentrations of Cu2+ (0 mg/L, 0.025 mg/L, 0.1 mg/L, 0.4 mg/L, 1.6 mg/L) in serum-free DMEM. Culture medium was collected in 12 h, 24 h, 36 h thereafter for GH measurement and the radioimmunoassay kits were provided by Linco.</p><p><b>RESULTS</b>The GH concentration was higher in treatments of 0.025 mg/L, 0.1 mg/L, 0.4 mg/L than that of 0 mg/L when the cells were treated with Cu2+ for 24 h, and it was significantly different (P < 0.05) between treatment 0.1 mg/L and treatment 0 mg/L.</p><p><b>CONCLUSION</b>It was concluded that the presence of Cu2+ stimulated pig pituitary cells to secret GH in vivo.</p>